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l lg ythdf1 proteintech  (Proteintech)


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    Proteintech l lg ythdf1 proteintech
    L Lg Ythdf1 Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 367 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Figure 5. The <t>m6A/YTHDF1/APC</t> pathway is required for axonal growth in cultured neurons and callosal neurons in vivo (A) Representative DIV6 neurons expressing shControl, shYthdf1, shMettl14, or shApc along with GFP. Scale bar, 50 μm. (B) Quantification of the longest axonal branch length (number of axons: shControl, n = 48; shYthdf1, n = 13; shMettl14, n = 16; shApc, n = 16) and the total number of axonal branch tips (shControl, n = 47; shYthdf1, n = 13; shMettl14; n = 16; shApc, n = 16). The results are normalized to the average of the shControl.
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    Figure 5. The <t>m6A/YTHDF1/APC</t> pathway is required for axonal growth in cultured neurons and callosal neurons in vivo (A) Representative DIV6 neurons expressing shControl, shYthdf1, shMettl14, or shApc along with GFP. Scale bar, 50 μm. (B) Quantification of the longest axonal branch length (number of axons: shControl, n = 48; shYthdf1, n = 13; shMettl14, n = 16; shApc, n = 16) and the total number of axonal branch tips (shControl, n = 47; shYthdf1, n = 13; shMettl14; n = 16; shApc, n = 16). The results are normalized to the average of the shControl.
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    Figure 5. The m6A/YTHDF1/APC pathway is required for axonal growth in cultured neurons and callosal neurons in vivo (A) Representative DIV6 neurons expressing shControl, shYthdf1, shMettl14, or shApc along with GFP. Scale bar, 50 μm. (B) Quantification of the longest axonal branch length (number of axons: shControl, n = 48; shYthdf1, n = 13; shMettl14, n = 16; shApc, n = 16) and the total number of axonal branch tips (shControl, n = 47; shYthdf1, n = 13; shMettl14; n = 16; shApc, n = 16). The results are normalized to the average of the shControl.

    Journal: Cell reports

    Article Title: m 6 A RNA methylation-mediated control of global APC expression is required for local translation of β-actin and axon development.

    doi: 10.1016/j.celrep.2025.115727

    Figure Lengend Snippet: Figure 5. The m6A/YTHDF1/APC pathway is required for axonal growth in cultured neurons and callosal neurons in vivo (A) Representative DIV6 neurons expressing shControl, shYthdf1, shMettl14, or shApc along with GFP. Scale bar, 50 μm. (B) Quantification of the longest axonal branch length (number of axons: shControl, n = 48; shYthdf1, n = 13; shMettl14, n = 16; shApc, n = 16) and the total number of axonal branch tips (shControl, n = 47; shYthdf1, n = 13; shMettl14; n = 16; shApc, n = 16). The results are normalized to the average of the shControl.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-YTHDF1 Proteintech Cat#:17479-1-AP; RRID:AB_2217473 Anti -YTHDF2 Proteintech Cat#:24744-1-AP; RRID:AB_2687435 Anti-YTHDF3 Santa Cruz Biotech Cat#:Sc-377119; RRID:AB_2687436 Anti-METTL14 ATLAS ANTIBODIES (Sigma) Cat#:HPA038002; RRID:AB_10672401 Anti-Tubulin (Tyrosinated) Abcam Cat#:ab6160; RRID:AB_305328 Anti-APC Millipore Cat#:OP80; RRID:AB_2057371 Anti-APC-C (C-20) Santa Cruz Cat#:SC-896; RRID:AB_2057493 Anti-FMRP Abcam Cat#:ab17722; RRID:AB_2278530 Anti-HA-tag MBL Cat#:M180-3; RRID:AB_10951811 Anti-HA tag Abcam Cat#:ab9110; RRID:AB_307019 Anti-GAPDH (14C10) Cell Signaling Cat#:2118; RRID:AB_561053 Anti-Puromycin Millipore Cat#:MABE343; RRID:AB_2566826 Anti-DCX Abcam Cat#:ab18723; RRID:AB_732011 Anti-CRMP2 Biolegend Cat#:869001; RRID:AB_732011 Anti-GFP Abcam Cat#:ab13970; RRID:AB_300798 Anti-Tau Abcam Cat#:ab75714; RRID:AB_1310734 Anti-MAP2 Abcam Cat#:ab5392; RRID:AB_2138153 Anti-Beta-actin Cayman Chemicals Cat#:32127; RRID:AB_2810170 Anti-Cre Recombinase Millipore Cat#:MAB3120; RRID:AB_2085748 Anti-Tubb2b Abcepta Cat#:AP11940a; RRID:AB_10820514 Goat Anti-Mouse IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 488 Thermofisher Cat#:A11001; RRID:AB_2534069 Goat Anti-Mouse IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 568 Thermofisher Cat#:A11004; RRID:AB_2534072 Goat Anti-Mouse IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 633 Thermofisher Cat#:A21050; RRID:AB_2535718 Goat Anti-Rabbit IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 633 Thermofisher Cat#:A21070; RRID:AB_2535731 Donkey anti-Chicken IgY (H + L) Highly Cross Adsorbed Secondary Antibody, Alexa Fluo 568 Thermofisher Cat# A78950; RRID:AB_2921072 Bacterial and virus strains NEB Stable Competent E. coli New England Biolabs Cat#:C3040H Chemicals, peptides, and recombinant proteins Puromycin InvivoGen Cat#:58-58-2 ProLong Diamond antifade mountant Thermofisher Cat#:P36965 DPBS Thermofisher Cat#:10010023 Neurobasal medium Thermofisher Cat#:10888022 MEM Thermofisher Cat#:11095080 Penicillin/Streptomycin Thermofisher Cat#:15140122 (Continued on next page) 18 Cell Reports 44, 115727, June 24, 2025

    Techniques: Cell Culture, In Vivo, Expressing

    Figure 7. Model depicting the regulation of axon development via the m6A/YTHDF1/ APC pathway, which governs β-actin mRNA transport and local translation

    Journal: Cell reports

    Article Title: m 6 A RNA methylation-mediated control of global APC expression is required for local translation of β-actin and axon development.

    doi: 10.1016/j.celrep.2025.115727

    Figure Lengend Snippet: Figure 7. Model depicting the regulation of axon development via the m6A/YTHDF1/ APC pathway, which governs β-actin mRNA transport and local translation

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-YTHDF1 Proteintech Cat#:17479-1-AP; RRID:AB_2217473 Anti -YTHDF2 Proteintech Cat#:24744-1-AP; RRID:AB_2687435 Anti-YTHDF3 Santa Cruz Biotech Cat#:Sc-377119; RRID:AB_2687436 Anti-METTL14 ATLAS ANTIBODIES (Sigma) Cat#:HPA038002; RRID:AB_10672401 Anti-Tubulin (Tyrosinated) Abcam Cat#:ab6160; RRID:AB_305328 Anti-APC Millipore Cat#:OP80; RRID:AB_2057371 Anti-APC-C (C-20) Santa Cruz Cat#:SC-896; RRID:AB_2057493 Anti-FMRP Abcam Cat#:ab17722; RRID:AB_2278530 Anti-HA-tag MBL Cat#:M180-3; RRID:AB_10951811 Anti-HA tag Abcam Cat#:ab9110; RRID:AB_307019 Anti-GAPDH (14C10) Cell Signaling Cat#:2118; RRID:AB_561053 Anti-Puromycin Millipore Cat#:MABE343; RRID:AB_2566826 Anti-DCX Abcam Cat#:ab18723; RRID:AB_732011 Anti-CRMP2 Biolegend Cat#:869001; RRID:AB_732011 Anti-GFP Abcam Cat#:ab13970; RRID:AB_300798 Anti-Tau Abcam Cat#:ab75714; RRID:AB_1310734 Anti-MAP2 Abcam Cat#:ab5392; RRID:AB_2138153 Anti-Beta-actin Cayman Chemicals Cat#:32127; RRID:AB_2810170 Anti-Cre Recombinase Millipore Cat#:MAB3120; RRID:AB_2085748 Anti-Tubb2b Abcepta Cat#:AP11940a; RRID:AB_10820514 Goat Anti-Mouse IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 488 Thermofisher Cat#:A11001; RRID:AB_2534069 Goat Anti-Mouse IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 568 Thermofisher Cat#:A11004; RRID:AB_2534072 Goat Anti-Mouse IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 633 Thermofisher Cat#:A21050; RRID:AB_2535718 Goat Anti-Rabbit IgG (H + L) cross-adsorbed secondary antibody Alexa Fluor 633 Thermofisher Cat#:A21070; RRID:AB_2535731 Donkey anti-Chicken IgY (H + L) Highly Cross Adsorbed Secondary Antibody, Alexa Fluo 568 Thermofisher Cat# A78950; RRID:AB_2921072 Bacterial and virus strains NEB Stable Competent E. coli New England Biolabs Cat#:C3040H Chemicals, peptides, and recombinant proteins Puromycin InvivoGen Cat#:58-58-2 ProLong Diamond antifade mountant Thermofisher Cat#:P36965 DPBS Thermofisher Cat#:10010023 Neurobasal medium Thermofisher Cat#:10888022 MEM Thermofisher Cat#:11095080 Penicillin/Streptomycin Thermofisher Cat#:15140122 (Continued on next page) 18 Cell Reports 44, 115727, June 24, 2025

    Techniques: